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Streamlined protein expression and purification using cleavable self-aggregating tags

BACKGROUND: Recombinant protein expression and purification remains a fundamental issue for biotechnology. Recently we found that two short self-assembling amphipathic peptides 18A (EWLKAFYEKVLEKLKELF) and ELK16 (LELELKLKLELELKLK) can induce the formation of active protein aggregates in Escherichia...

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Detalhes bibliográficos
Main Authors: Xing, Lei, Wu, Wei, Zhou, Bihong, Lin, Zhanglin
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3124420/
https://ncbi.nlm.nih.gov/pubmed/21631955
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-10-42
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