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Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice

Fluorescent proteins (FPs) have great utility in identifying specific cell populations and in studying cellular dynamics in the mouse. To quantify the factors that determine both the expression and relative brightness of FPs in mouse embryonic stem cells (mESCs) and in mice, we generated eight diffe...

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Autori principali: Chen, Sara X., Osipovich, Anna B., Ustione, Alessandro, Potter, Leah A., Hipkens, Susan, Gangula, Rama, Yuan, Weiping, Piston, David W., Magnuson, Mark A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The Company of Biologists Limited 2011
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3124063/
https://ncbi.nlm.nih.gov/pubmed/21324933
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dmm.006569
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