Modulation of Functionally Significant Conformational Equilibria in Adenylate Kinase by High Concentrations of Trimethylamine Oxide Attributed to Volume Exclusion

The effect of an inert small molecule osmolyte, trimethyl amine N-oxide (TMAO), upon the conformational equilibria of Escherichia coli adenylate kinase was studied using time-resolved FRET. The relative populations of open and closed clefts between the LID and the CORE domains were measured as funct...

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Detalhes bibliográficos
Main Authors: Nagarajan, Sureshbabu, Amir, Dan, Grupi, Asaf, Goldenberg, David P., Minton, Allen P., Haas, Elisha
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2011
Assuntos:
Protein
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3123985/
https://ncbi.nlm.nih.gov/pubmed/21689533
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2011.03.065
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