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Site-directed and transposon-mediated mutagenesis with pfd-plasmids by electroporation of Erwinia amylovora and Escherichia coli cells.

The suicide plasmid pfdA31-Tn5 was constructed to mutagenize Erwinia amylovora and Escherichia coli strains by electorporation. This vector carries the bacteriophage fd replication origin, a beta-lactamase gene and the transposon Tn5. For propagation the plasmid depends on host cells producing fd ge...

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Bibliografske podrobnosti
izdano v:Nucleic Acids Res
Main Authors: Metzger, M, Bellemann, P, Schwartz, T, Geider, K
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 1992
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC312340/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1317549/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.9.2265
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