Site-directed and transposon-mediated mutagenesis with pfd-plasmids by electroporation of Erwinia amylovora and Escherichia coli cells.

The suicide plasmid pfdA31-Tn5 was constructed to mutagenize Erwinia amylovora and Escherichia coli strains by electorporation. This vector carries the bacteriophage fd replication origin, a beta-lactamase gene and the transposon Tn5. For propagation the plasmid depends on host cells producing fd ge...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Metzger, M, Bellemann, P, Schwartz, T, Geider, K
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1992
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC312340/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1317549/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.9.2265
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