Modifications of the E.coli Lac repressor for expression in eukaryotic cells: effects of nuclear signal sequences on protein activity and nuclear accumulation.
Eukaryotic expression vectors designed to produce E. coli Lac repressor protein targeted to the nucleus of mammalian cells were constructed. These constructions carry the lac repressor gene (lacI) fused at different positions to a nuclear localization sequence (NLS) from either the SV40 large T anti...
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| Pubblicato in: | Nucleic Acids Res |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
1992
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| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC312271/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1315957/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.7.1785 |
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