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Functional Expression of Spider Neurotoxic Peptide Huwentoxin-I in E. coli

The coding sequence of huwentoxin-I, a neurotoxic peptide isolated from the venom of the Chinese spider Ornithoctonus huwena, was amplified by PCR using the cDNA library constructed from the spider venom glands. The cloned fragment was inserted into the expression vector pET-40b and transformed into...

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Bibliografiske detaljer
Main Authors: Meng, Er, Cai, Tian-Fu, Li, Wen-Ying, Zhang, Hui, Liu, Yan-Bo, Peng, Kuan, Liang, Songping, Zhang, Dong-Yi
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3121796/
https://ncbi.nlm.nih.gov/pubmed/21731778
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0021608
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