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Watching Individual Proteins Acting on Single Molecules of DNA

In traditional biochemical experiments, the behavior of individual proteins is obscured by ensemble averaging. To better understand the behavior of proteins that bind to and/or translocate on DNA, we have developed instrumentation that uses optical trapping, microfluidic solution delivery, and fluor...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Amitani, Ichiro, Liu, Bian, Dombrowski, Christopher C., Baskin, Ronald J., Kowalczykowski, Stephen C.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3119597/
https://ncbi.nlm.nih.gov/pubmed/20580968
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0076-6879(10)72007-3
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