Analysis of nuclear factor I binding to DNA using degenerate oligonucleotides.
Nuclear factor I (NFI) binds tightly to DNA containing the consensus sequence TGG(N)6-7GCCAA. To study the role of the spacing between the TGG and GCCAA motifs, oligonucleotides homologous to the NFI binding site FIB-2 were synthesized and used for binding assays in vitro. The wild-type site (FIB-2....
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| Publicado no: | Nucleic Acids Res |
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| Autor principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1986
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311933/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3786147/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.22.9117 |
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