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Analysis of nuclear factor I binding to DNA using degenerate oligonucleotides.
Nuclear factor I (NFI) binds tightly to DNA containing the consensus sequence TGG(N)6-7GCCAA. To study the role of the spacing between the TGG and GCCAA motifs, oligonucleotides homologous to the NFI binding site FIB-2 were synthesized and used for binding assays in vitro. The wild-type site (FIB-2....
Kaydedildi:
| Yazar: | |
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| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
1986
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| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC311933/ https://ncbi.nlm.nih.gov/pubmed/3786147 |
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