Site directed mutagenesis experiments suggest that Glu 111, Glu 144 and Arg 145 are essential for endonucleolytic activity of EcoRI.

We have constructed a plasmid (pRIF 309+) carrying the EcoRI restriction endonuclease gene and the f1 origin of replication. Upon transformation of this plasmid into E. coli and infection with bacteriophage f1 single stranded plasmids are produced which can be used for sequencing and site directed m...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Wolfes, H, Alves, J, Fliess, A, Geiger, R, Pingoud, A
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1986
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311929/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3024128/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.22.9063
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