Site directed mutagenesis experiments suggest that Glu 111, Glu 144 and Arg 145 are essential for endonucleolytic activity of EcoRI.

Lignende værker

• Role of thymidine residues in DNA recognition by the EcoRI and EcoRV restriction endonucleases.
  af: Fliess, A, et al.
  Udgivet: (1986)
• Substrate-assisted catalysis in the cleavage of DNA by the EcoRI and EcoRV restriction enzymes.
  af: Jeltsch, A, et al.
  Udgivet: (1993)
• Does the specific recognition of DNA by the restriction endonuclease EcoRI involve a linear diffusion step? Investigation of the processivity of the EcoRI endonuclease.
  af: Langowski, J, et al.
  Udgivet: (1983)
• Heparin inhibits EcoRI endonuclease cleavage of DNA at certain EcoRI sites.
  af: Chen, J Z, et al.
  Udgivet: (1990)
• EcoRI cleavage sites in the argECBH region of the Escherichia coli chromosome.
  af: Devine, E A, et al.
  Udgivet: (1977)