Altered DNA conformations in the gene regulatory region of torsionally-stressed SV40 DNA.
We used mung bean nuclease to probe the SV40 genome for DNA unwinding and unpairing. Cleavage occurred at a limited number of specific sites in supercoiled, but not relaxed DNA. The number and location of cleavage sites depended upon Mg2+ concentration. Without Mg2+, cutting occurred mainly in one e...
Guardat en:
| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1986
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311922/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3024125/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.22.8949 |
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