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Altered DNA conformations in the gene regulatory region of torsionally-stressed SV40 DNA.

We used mung bean nuclease to probe the SV40 genome for DNA unwinding and unpairing. Cleavage occurred at a limited number of specific sites in supercoiled, but not relaxed DNA. The number and location of cleavage sites depended upon Mg2+ concentration. Without Mg2+, cutting occurred mainly in one e...

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Bibliografische gegevens
Hoofdauteurs: Iacono-Connors, L, Kowalski, D
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1986
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC311922/
https://ncbi.nlm.nih.gov/pubmed/3024125
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