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Altered DNA conformations in the gene regulatory region of torsionally-stressed SV40 DNA.
We used mung bean nuclease to probe the SV40 genome for DNA unwinding and unpairing. Cleavage occurred at a limited number of specific sites in supercoiled, but not relaxed DNA. The number and location of cleavage sites depended upon Mg2+ concentration. Without Mg2+, cutting occurred mainly in one e...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1986
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC311922/ https://ncbi.nlm.nih.gov/pubmed/3024125 |
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