Efficient construction of cDNA libraries in plasmid expression vectors using an adaptor strategy.
We describe a method for the construction of large DNA fragment libraries in plasmid vectors, in which complementary, single-stranded extensions are ligated onto both vector and insert DNA using un-phosphorylated adaptor oligonucleotides. Special consideration has been taken of the requirements of e...
Sábháilte in:
| Foilsithe in: | Nucleic Acids Res |
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| Príomhchruthaitheoirí: | , , , , |
| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe / Cruthaithe: |
Oxford University Press
1986
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| Rochtain ar líne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311881/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3024111/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.21.8615 |
| Clibeanna: |
Níl clibeanna ann, Bí ar an gcéad duine le clib a chur leis an taifead seo!
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