Efficient construction of cDNA libraries in plasmid expression vectors using an adaptor strategy.
We describe a method for the construction of large DNA fragment libraries in plasmid vectors, in which complementary, single-stranded extensions are ligated onto both vector and insert DNA using un-phosphorylated adaptor oligonucleotides. Special consideration has been taken of the requirements of e...
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| Опубликовано в:: | Nucleic Acids Res |
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| Главные авторы: | , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Oxford University Press
1986
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| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311881/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3024111/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.21.8615 |
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