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Efficient construction of cDNA libraries in plasmid expression vectors using an adaptor strategy.
We describe a method for the construction of large DNA fragment libraries in plasmid vectors, in which complementary, single-stranded extensions are ligated onto both vector and insert DNA using un-phosphorylated adaptor oligonucleotides. Special consideration has been taken of the requirements of e...
Guardado en:
| Autores principales: | , , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
1986
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| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC311881/ https://ncbi.nlm.nih.gov/pubmed/3024111 |
| Etiquetas: |
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