Efficient construction of cDNA libraries in plasmid expression vectors using an adaptor strategy.

We describe a method for the construction of large DNA fragment libraries in plasmid vectors, in which complementary, single-stranded extensions are ligated onto both vector and insert DNA using un-phosphorylated adaptor oligonucleotides. Special consideration has been taken of the requirements of e...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Haymerle, H, Herz, J, Bressan, G M, Frank, R, Stanley, K K
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1986
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311881/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3024111/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.21.8615
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