Nalaganje...
Efficient construction of cDNA libraries in plasmid expression vectors using an adaptor strategy.
We describe a method for the construction of large DNA fragment libraries in plasmid vectors, in which complementary, single-stranded extensions are ligated onto both vector and insert DNA using un-phosphorylated adaptor oligonucleotides. Special consideration has been taken of the requirements of e...
Shranjeno v:
| Main Authors: | , , , , |
|---|---|
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
1986
|
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC311881/ https://ncbi.nlm.nih.gov/pubmed/3024111 |
| Oznake: |
Označite
Brez oznak, prvi označite!
|