Загрузка...

Efficient construction of cDNA libraries in plasmid expression vectors using an adaptor strategy.

We describe a method for the construction of large DNA fragment libraries in plasmid vectors, in which complementary, single-stranded extensions are ligated onto both vector and insert DNA using un-phosphorylated adaptor oligonucleotides. Special consideration has been taken of the requirements of e...

Полное описание

Сохранить в:
Библиографические подробности
Главные авторы: Haymerle, H, Herz, J, Bressan, G M, Frank, R, Stanley, K K
Формат: Artigo
Язык:Inglês
Опубликовано: 1986
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC311881/
https://ncbi.nlm.nih.gov/pubmed/3024111
Метки: Добавить метку
Нет меток, Требуется 1-ая метка записи!