A high-throughput immobilized bead screen for stable proteins and multi-protein complexes

We describe an in vitro colony screen to identify Escherichia coli expressing soluble proteins and stable, assembled multiprotein complexes. Proteins with an N-terminal 6His tag and C-terminal green fluorescent protein (GFP) S11 tag are fluorescently labeled in cells by complementation with a coexpr...

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Main Authors: Lockard, Meghan A., Listwan, Pawel, Pedelacq, Jean-Denis, Cabantous, Stéphanie, Nguyen, Hau B., Terwilliger, Thomas C., Waldo, Geoffrey S.
格式: Artigo
語言:Inglês
出版: Oxford University Press 2011
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Original Articles
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3118733/
https://ncbi.nlm.nih.gov/pubmed/21642284
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzr021
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