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A high-throughput immobilized bead screen for stable proteins and multi-protein complexes
We describe an in vitro colony screen to identify Escherichia coli expressing soluble proteins and stable, assembled multiprotein complexes. Proteins with an N-terminal 6His tag and C-terminal green fluorescent protein (GFP) S11 tag are fluorescently labeled in cells by complementation with a coexpr...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3118733/ https://ncbi.nlm.nih.gov/pubmed/21642284 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzr021 |
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