Učitavanje...
Chromogenic identification of oligonucleotide-directed mutants.
We describe a simple plaque color assay for identifying oligonucleotide-directed mutations in cloned DNA fragments. The basis of the method is to: fuse the sequence of interest in-frame to the E.coli lacZ gene to produce a blue plaque phage, mutate the site of interest to a stop codon to generate a...
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| Glavni autori: | , , |
|---|---|
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
1986
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| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC311872/ https://ncbi.nlm.nih.gov/pubmed/3537959 |
| Oznake: |
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