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Chromogenic identification of oligonucleotide-directed mutants.
We describe a simple plaque color assay for identifying oligonucleotide-directed mutations in cloned DNA fragments. The basis of the method is to: fuse the sequence of interest in-frame to the E.coli lacZ gene to produce a blue plaque phage, mutate the site of interest to a stop codon to generate a...
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| Hlavní autoři: | , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
1986
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| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC311872/ https://ncbi.nlm.nih.gov/pubmed/3537959 |
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