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Cloning the DdeI restriction-modification system using a two-step method.
DdeI, a Type II restriction-modification system from the gram-negative anaerobic bacterium Desulfovibrio desulfuricans, recognizes the sequence CTNAG. The system has been cloned into E. coli in two steps. First the methylase gene was cloned into pBR322 and a derivative expressing higher levels was c...
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| Hlavní autoři: | , , , , , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
1986
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| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC311826/ https://ncbi.nlm.nih.gov/pubmed/3022241 |
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