Cloning the DdeI restriction-modification system using a two-step method.
DdeI, a Type II restriction-modification system from the gram-negative anaerobic bacterium Desulfovibrio desulfuricans, recognizes the sequence CTNAG. The system has been cloned into E. coli in two steps. First the methylase gene was cloned into pBR322 and a derivative expressing higher levels was c...
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| Yayımlandı: | Nucleic Acids Res |
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| Asıl Yazarlar: | , , , , , , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Oxford University Press
1986
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| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311826/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3022241/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.20.7939 |
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