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Cloning the DdeI restriction-modification system using a two-step method.

DdeI, a Type II restriction-modification system from the gram-negative anaerobic bacterium Desulfovibrio desulfuricans, recognizes the sequence CTNAG. The system has been cloned into E. coli in two steps. First the methylase gene was cloned into pBR322 and a derivative expressing higher levels was c...

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Bibliografske podrobnosti
Main Authors: Howard, K A, Card, C, Benner, J S, Callahan, H L, Maunus, R, Silber, K, Wilson, G, Brooks, J E
Format: Artigo
Jezik:Inglês
Izdano: 1986
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC311826/
https://ncbi.nlm.nih.gov/pubmed/3022241
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