Cloning the DdeI restriction-modification system using a two-step method.

DdeI, a Type II restriction-modification system from the gram-negative anaerobic bacterium Desulfovibrio desulfuricans, recognizes the sequence CTNAG. The system has been cloned into E. coli in two steps. First the methylase gene was cloned into pBR322 and a derivative expressing higher levels was c...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Howard, K A, Card, C, Benner, J S, Callahan, H L, Maunus, R, Silber, K, Wilson, G, Brooks, J E
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 1986
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC311826/
https://ncbi.nlm.nih.gov/pubmed/3022241
Tagiau: Ychwanegu Tag
Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!

Eitemau Tebyg