Cloning the DdeI restriction-modification system using a two-step method.

DdeI, a Type II restriction-modification system from the gram-negative anaerobic bacterium Desulfovibrio desulfuricans, recognizes the sequence CTNAG. The system has been cloned into E. coli in two steps. First the methylase gene was cloned into pBR322 and a derivative expressing higher levels was c...

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Publicado en:	Nucleic Acids Res
Autores principales:	Howard, K A, Card, C, Benner, J S, Callahan, H L, Maunus, R, Silber, K, Wilson, G, Brooks, J E
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	Oxford University Press 1986
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311826/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3022241/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.20.7939
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