Application of 2-cyanoethyl N,N,N',N'-tetraisopropylphosphorodiamidite for in situ preparation of deoxyribonucleoside phosphoramidites and their use in polymer-supported synthesis of oligodeoxyribonucleotides.

Deoxyribonucleoside phosphoramidites are prepared in situ from 5'-O,N-protected deoxyribonucleosides and 2-cyanoethyl N,N,N',N'-tetraisopropylphosphorodiamidite with tetrazole as catalyst, and the solutions applied directly on an automatic solid-phase DNA synthesizer. Using LCAA-CPG s...

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Veröffentlicht in:Nucleic Acids Res
Hauptverfasser: Nielsen, J, Taagaard, M, Marugg, J E, van Boom, J H, Dahl, O
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 1986
Online Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311758/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3763407/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.18.7391
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