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A non-radioactive in situ hybridization method based on mercurated nucleic acid probes and sulfhydryl-hapten ligands.

Mercurated nucleic acid probes can be used for non-radioactive in situ hybridization. The principle of the method is based on the reaction of the mercurated pyrimidine residues of the in situ hybridized probe with the sulfhydryl group of a ligand which contains a hapten. Next, the hapten is immunocy...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Hopman, A H, Wiegant, J, Tesser, G I, Van Duijn, P
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 1986
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311658/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3748817/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.16.6471
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