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Improved In Situ Hybridization Efficiency with Locked-Nucleic-Acid-Incorporated DNA Probes
Low signal intensity due to poor probe hybridization efficiency is one of the major drawbacks of rRNA-targeted in situ hybridization. There are two major factors affecting the hybridization efficiency: probe accessibility and affinity to the targeted rRNA molecules. In this study, we demonstrate rem...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology
2006
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1538721/ https://ncbi.nlm.nih.gov/pubmed/16885281 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03039-05 |
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