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Improved In Situ Hybridization Efficiency with Locked-Nucleic-Acid-Incorporated DNA Probes

Low signal intensity due to poor probe hybridization efficiency is one of the major drawbacks of rRNA-targeted in situ hybridization. There are two major factors affecting the hybridization efficiency: probe accessibility and affinity to the targeted rRNA molecules. In this study, we demonstrate rem...

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Detalhes bibliográficos
Main Authors: Kubota, Kengo, Ohashi, Akiyoshi, Imachi, Hiroyuki, Harada, Hideki
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1538721/
https://ncbi.nlm.nih.gov/pubmed/16885281
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03039-05
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