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The minimal duplex DNA sequence required for site-specific recombination promoted by the FLP protein of yeast in vitro.
The 2-micron plasmid of the yeast Saccharomyces cerevisiae codes for a site-specific recombinase ('FLP') that efficiently catalyses recombination across the plasmid's two 599 bp repeats both in vivo and in vitro. We have used the partially purified FLP protein to define the minimal du...
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| Main Authors: | , , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1986
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC311492/ https://ncbi.nlm.nih.gov/pubmed/3523436 |
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