A novel method for the determination of post-transcriptional modification in RNA by mass spectrometry.

A method is described for the detection, chemical characterization and sequence placement of post-transcriptionally modified nucleotides in RNA. Molecular masses of oligonucleotides produced by RNase T1 hydrolysis can be measured by electrospray mass spectrometry with errors of less than 1 Da, which...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Kowalak, J A, Pomerantz, S C, Crain, P F, McCloskey, J A
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1993
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311193/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8233793/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.19.4577
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