Benchmarking Membrane Protein Detergent Stability for Improving Throughput of High-Resolution X-ray Structures

Obtaining well-ordered crystals is a major hurdle to X-ray structure determination of membrane proteins. To facilitate crystal optimization, we investigated the detergent stability of 24 eukaryotic and prokaryotic membrane proteins, predominantly transporters, using a fluorescent-based unfolding ass...

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Detalhes bibliográficos
Main Authors: Sonoda, Yo, Newstead, Simon, Hu, Nien-Jen, Alguel, Yilmaz, Nji, Emmanuel, Beis, Konstantinos, Yashiro, Shoko, Lee, Chiara, Leung, James, Cameron, Alexander D., Byrne, Bernadette, Iwata, So, Drew, David
Formato: Artigo
Idioma:Inglês
Publicado em: Cell Press 2011
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Ways & Means
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3111809/
https://ncbi.nlm.nih.gov/pubmed/21220112
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.str.2010.12.001
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