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GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae

It is often difficult to produce eukaryotic membrane proteins in large quantities, which is a major obstacle for analyzing their biochemical and structural features. To date, yeast has been the most successful heterologous overexpression system in producing eukaryotic membrane proteins for high-reso...

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Bibliografski detalji
Glavni autori: Drew, David, Newstead, Simon, Sonoda, Yo, Kim, Hyun, von Heijne, Gunnar, Iwata, So
Format: Artigo
Jezik:Inglês
Izdano: 2008
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2744353/
https://ncbi.nlm.nih.gov/pubmed/18451787
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2008.44
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