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High-Throughput SNP Allele-Frequency Determination in Pooled DNA Samples by Kinetic PCR

We have developed an accurate, yet inexpensive and high-throughput, method for determining the allele frequency of biallelic polymorphisms in pools of DNA samples. The assay combines kinetic (real-time quantitative) PCR with allele-specific amplification and requires no post-PCR processing. The rela...

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Autori principali: Germer, Søren, Holland, Michael J., Higuchi, Russell
Natura: Artigo
Lingua:Inglês
Pubblicazione: Cold Spring Harbor Laboratory Press 2000
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC310828/
https://ncbi.nlm.nih.gov/pubmed/10673283
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