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Measurement of protein unfolding/refolding kinetics and structural characterization of hidden intermediates by NMR relaxation dispersion

Detailed understanding of protein function and malfunction hinges on the ability to characterize transiently populated states and the transitions between them. Here, we use (15)N, [Image: see text], and (13)CO NMR R(2) relaxation dispersion to investigate spontaneous unfolding and refolding events o...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Meinhold, Derrick W., Wright, Peter E.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: National Academy of Sciences 2011
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3107309/
https://ncbi.nlm.nih.gov/pubmed/21562212
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1105682108
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