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Purification, crystallization and X-ray characterization of a Kunitz-type trypsin inhibitor protein from the seeds of chickpea (Cicer arietinum)

A Kunitz-type trypsin inhibitor protein (CPTI) purified from chickpea seeds was estimated to have a molecular mass of 18 kDa on SDS–PAGE. The IC(50) value of CPTI was determined to be 2.5 µg against trypsin. The inhibitory activity of CPTI is 114 TIU (trypsin inhibitory units) per milligram of prote...

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Auteurs principaux: Sharma, Urvashi, Suresh, C. G.
Format: Artigo
Langue:Inglês
Publié: International Union of Crystallography 2011
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3107151/
https://ncbi.nlm.nih.gov/pubmed/21636920
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111015338
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