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Efficient selection of 3'-terminal exons from vertebrate DNA.
Identification of expressed sequences within genomic DNA is a hurdle in the characterization of complex genomes. We developed an exon trapping scheme that provides a positive selection for vertebrate 3'-terminal exons. A copy of the trapped exon sequence is obtained by RT/PCR amplification. The...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1993
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC310637/ https://ncbi.nlm.nih.gov/pubmed/8255777 |
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