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Specific isolation of 3'-terminal exons of human genes by exon trapping.

Exon trapping is a method to functionally clone expressed sequences from genomic DNA. We have previously developed the vector system pETV-SD2, which contains only a splice donor site (SD) followed by a LacZ gene, allowing trapping of internal exons of human genes by blue-white selection. We now desc...

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Main Authors: Datson, N A, Duyk, G M, Van Ommen, J B, Den Dunnen, J T
格式: Artigo
語言:Inglês
出版: 1994
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC331902/
https://ncbi.nlm.nih.gov/pubmed/7937140
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