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In vivo cloning of PCR products in E. coli.
This report describes an efficient method to clone PCR products exploiting endogenous Escherichia coli enzymatic activities. PCR products are engineered to contain terminal sequences identical to sequences at the two ends of a linearized vector. PCR products and vector DNA are then simply co-transfe...
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| 主要な著者: | , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
1993
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| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC310636/ https://ncbi.nlm.nih.gov/pubmed/8255776 |
| タグ: |
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