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The heat-shock DnaK protein is required for plasmid R1 replication and it is dispensable for plasmid ColE1 replication.
Plasmid R1 replication in vitro is inactive in extracts prepared from a dnaK756 strain but is restored to normal levels upon addition of purified DnaK protein. Replication of R1 in extracts of a dnaKwt strain can be specifically inhibited with polyclonal antibodies against DnaK. RepA-dependent repli...
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| Auteurs principaux: | , , , , |
|---|---|
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
1993
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| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC310592/ https://ncbi.nlm.nih.gov/pubmed/8265367 |
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