The heat-shock DnaK protein is required for plasmid R1 replication and it is dispensable for plasmid ColE1 replication.

Plasmid R1 replication in vitro is inactive in extracts prepared from a dnaK756 strain but is restored to normal levels upon addition of purified DnaK protein. Replication of R1 in extracts of a dnaKwt strain can be specifically inhibited with polyclonal antibodies against DnaK. RepA-dependent repli...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Giraldo-Suárez, R, Fernández-Tresguerres, E, Díaz-Orejas, R, Malki, A, Kohiyama, M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1993
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310592/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8265367/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.23.5495
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