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Separation of mutant and wild-type ribosomes based on differences in their anti Shine-Dalgarno sequence.

We describe a system to isolate 30S ribosomal subunits which contain targeted mutations in their 16S rRNA. The mutations of interest should be present in so-called specialized 30S subunits which have an anti-Shine-Dalgarno sequence that is altered from 5' ACCUCC to 5' ACACAC. These plasmid-encoded s...

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Udgivet i:Nucleic Acids Res
Principais autores: Poot, R A, Brink, M F, Pleij, C W, de Boer, H A, van Duin, J
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1993
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310577/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8265355/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.23.5398
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