Separation of mutant and wild-type ribosomes based on differences in their anti Shine-Dalgarno sequence.
We describe a system to isolate 30S ribosomal subunits which contain targeted mutations in their 16S rRNA. The mutations of interest should be present in so-called specialized 30S subunits which have an anti-Shine-Dalgarno sequence that is altered from 5' ACCUCC to 5' ACACAC. These plasmid-encoded s...
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| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1993
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| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310577/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8265355/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.23.5398 |
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