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Separation of mutant and wild-type ribosomes based on differences in their anti Shine-Dalgarno sequence.

We describe a system to isolate 30S ribosomal subunits which contain targeted mutations in their 16S rRNA. The mutations of interest should be present in so-called specialized 30S subunits which have an anti-Shine-Dalgarno sequence that is altered from 5' ACCUCC to 5' ACACAC. These plasmid...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Poot, R A, Brink, M F, Pleij, C W, de Boer, H A, van Duin, J
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 1993
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC310577/
https://ncbi.nlm.nih.gov/pubmed/8265355
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