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Separation of mutant and wild-type ribosomes based on differences in their anti Shine-Dalgarno sequence.
We describe a system to isolate 30S ribosomal subunits which contain targeted mutations in their 16S rRNA. The mutations of interest should be present in so-called specialized 30S subunits which have an anti-Shine-Dalgarno sequence that is altered from 5' ACCUCC to 5' ACACAC. These plasmid...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1993
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC310577/ https://ncbi.nlm.nih.gov/pubmed/8265355 |
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