Heterologous Overexpression and Mutagenesis of the Human Bile Salt Export Pump (ABCB11) Using DREAM (Directed REcombination-Assisted Mutagenesis)

Homologous recombination in Saccharomyces cerevisiae is a well-studied process. Here, we describe a yeast-recombination-based approach to construct and mutate plasmids containing the cDNA of the human bile salt export pump (BSEP) that has been shown to be unstable in E. coli. Using this approach, we...

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Detalhes bibliográficos
Main Authors: Stindt, Jan, Ellinger, Philipp, Stross, Claudia, Keitel, Verena, Häussinger, Dieter, Smits, Sander H. J., Kubitz, Ralf, Schmitt, Lutz
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2011
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3105083/
https://ncbi.nlm.nih.gov/pubmed/21655228
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0020562
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