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Rapid bacterial artificial chromosome modification for large-scale mouse transgenesis
We report here a high-throughput method for the modification of bacterial artificial chromosomes (BACs) that uses a novel two-plasmid approach. In this protocol, a vector modified in our laboratory to hold an R6Kγ origin of replication and a marker recombination cassette is inserted into a BAC in a...
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| Главные авторы: | , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2010
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3104474/ https://ncbi.nlm.nih.gov/pubmed/20885380 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2010.131 |
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