PCR using a thermostable polymerase with 3' to 5' exonuclease activity generates blunt products suitable for direct cloning.

Lignende værker

• Directional cloning of PCR products using exonuclease III.
  af: Kaluz, S, et al.
  Udgivet: (1992)
• Direct ligation of PCR products for cloning and sequencing.
  af: Haqqi, T M
  Udgivet: (1992)
• Exonuclease III induced ligase-free directional subcloning of PCR products.
  af: Hsiao, K
  Udgivet: (1993)
• Kinasing PCR Products for Efficient Blunt-End Cloning and Linker Addition
  af: Kanungo, Jyotshnabala, et al.
  Udgivet: (1993)
• A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products
  af: Motohashi, Ken
  Udgivet: (2019)