PCR using a thermostable polymerase with 3' to 5' exonuclease activity generates blunt products suitable for direct cloning.

Podobne knjige/članki

• Directional cloning of PCR products using exonuclease III.
  od: Kaluz, S, et al.
  Izdano: (1992)
• Direct ligation of PCR products for cloning and sequencing.
  od: Haqqi, T M
  Izdano: (1992)
• Exonuclease III induced ligase-free directional subcloning of PCR products.
  od: Hsiao, K
  Izdano: (1993)
• Kinasing PCR Products for Efficient Blunt-End Cloning and Linker Addition
  od: Kanungo, Jyotshnabala, et al.
  Izdano: (1993)
• Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9 degrees N-7 and mutations affecting 3'-5' exonuclease activity.
  od: Southworth, M W, et al.
  Izdano: (1996)