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Real Time FRET Based Detection of Mechanical Stress in Cytoskeletal and Extracellular Matrix Proteins

A molecular force sensing cassette (stFRET) was incorporated into actinin, filamin, and spectrin in vascular endothelial cells (BAECs) and into collagen-19 in Caenorhabditis elegans. To estimate the stress sensitivity of stFRET in solution, we used DNA springs. A 60-mer loop of single stranded DNA w...

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Bibliografiska uppgifter
Huvudupphovsmän: Meng, Fanjie, Suchyna, Thomas M., Lazakovitch, Elena, Gronostajski, Richard M., Sachs, Frederick
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2011
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3101475/
https://ncbi.nlm.nih.gov/pubmed/21625401
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12195-010-0140-0
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