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Real Time FRET Based Detection of Mechanical Stress in Cytoskeletal and Extracellular Matrix Proteins
A molecular force sensing cassette (stFRET) was incorporated into actinin, filamin, and spectrin in vascular endothelial cells (BAECs) and into collagen-19 in Caenorhabditis elegans. To estimate the stress sensitivity of stFRET in solution, we used DNA springs. A 60-mer loop of single stranded DNA w...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3101475/ https://ncbi.nlm.nih.gov/pubmed/21625401 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12195-010-0140-0 |
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