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Use of PCR primers containing a 3'-terminal ribose residue to prevent cross-contamination of amplified sequences.

Cross-contamination with previously amplified products poses a serious limitation in the use of PCR for clinical testing and in certain research applications as well. In the present study we report the use of novel primers containing a 3'-terminal ribose residue to circumvent this problem. Exte...

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Hlavní autoři: Walder, R Y, Hayes, J R, Walder, J A
Médium: Artigo
Jazyk:Inglês
Vydáno: 1993
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC310069/
https://ncbi.nlm.nih.gov/pubmed/8414989
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