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Influence of the three nucleotides upstream of the initiation codon on expression of the Escherichia coli lacZ gene in Saccharomyces cerevisiae.
By introducing synthetic oligonucleotides into a lacZ-yeast expression vector a set of 47 plasmids (out of 64 possible) was generated, differing only in the three bases immediately upstream of the AUG initiation codon of the Escherichia coli lacZ gene. Expression of the beta-galactosidase fusion pro...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1993
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| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310060/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8414981/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.18.4268 |
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