Detection of Infectious Adenovirus in Cell Culture by mRNA Reverse Transcription-PCR

We have developed and evaluated the reverse transcription (RT)-PCR detection of mRNA in cell culture to assay infectious adenoviruses (Ads) by using Ad type 2 (Ad2) and Ad41 as models. Only infectious Ads are detected because they are the only ones able to produce mRNA during replication in cell cul...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Ko, Gwangpyo, Cromeans, Theresa L., Sobsey, Mark D.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2003
Assuntos:
Public Health Microbiology
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC309946/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14660388/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.12.7377-7384.2003
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