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Detection of Infectious Adenovirus in Cell Culture by mRNA Reverse Transcription-PCR
We have developed and evaluated the reverse transcription (RT)-PCR detection of mRNA in cell culture to assay infectious adenoviruses (Ads) by using Ad type 2 (Ad2) and Ad41 as models. Only infectious Ads are detected because they are the only ones able to produce mRNA during replication in cell cul...
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| Главные авторы: | , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society for Microbiology
2003
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC309946/ https://ncbi.nlm.nih.gov/pubmed/14660388 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.12.7377-7384.2003 |
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