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Detection of Infectious Adenovirus in Cell Culture by mRNA Reverse Transcription-PCR

We have developed and evaluated the reverse transcription (RT)-PCR detection of mRNA in cell culture to assay infectious adenoviruses (Ads) by using Ad type 2 (Ad2) and Ad41 as models. Only infectious Ads are detected because they are the only ones able to produce mRNA during replication in cell cul...

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מידע ביבליוגרפי
Main Authors: Ko, Gwangpyo, Cromeans, Theresa L., Sobsey, Mark D.
פורמט: Artigo
שפה:Inglês
יצא לאור: American Society for Microbiology 2003
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC309946/
https://ncbi.nlm.nih.gov/pubmed/14660388
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.12.7377-7384.2003
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