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Study of polytopic membrane protein topological organization as a function of membrane lipid composition

A protocol is described using lipid mutants and thiol-specific chemical reagents to study lipid-dependent and host-specific membrane protein topogenesis by the substituted-cysteine accessibility method as applied to transmembrane domains (SCAM(TM)). SCAM(TM) is adapted to follow changes in membrane...

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Autores principales: Bogdanov, Mikhail, Heacock, Philip N., Dowhan, William
Formato: Artigo
Lenguaje:Inglês
Publicado: 2010
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3099133/
https://ncbi.nlm.nih.gov/pubmed/20419405
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60327-412-8_5
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