Homologous pairing between single-stranded DNA immobilized on a nitrocellulose membrane and duplex DNA is specific for RecA activity in bacterial crude extract.

Reaction between a circular single stranded and a linear double stranded DNA molecule (ssDNA and dsDNA) provides an efficient system to study recombination mediated by RecA protein. However, classical assays using reaction in solution require highly purified enzymes. This limits biochemical studies...

ver descrição completa

Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Bertrand, P, Corteggiani, E, Dutreix, M, Coppey, J, Lopez, B S
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1993
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC309861/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8367282/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.16.3653
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!